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In Vitro fertilisation may be the means of fertilising eggs away from body. It's commonly attempted in cases of infertility. In this technique, the eggs from a woman are taken through a needle and incubated with sperms from her partner or perhaps a donor. This results in fertilisation and the fertilised egg is transferred back once again to the uterus to grow.
What're the mandatory requirements for IVF?
IVF requires three basic necessities, a wholesome egg, a wholesome sperm and healthy uterus which can carry the pregnancy. This question is important because it is not necessary to have all three to make a bay. Let's for example consider women who are unable to produce eggs, but has an operating uterus. In this case egg donation is really a reasonable option. Similarly if sperms of the male partner are defective then donor sperms may also be used. This really is called surrogacy. There is another form of surrogacy; many women end up having their uterus which prevents them from carrying a pregnancy. The embryos produced by fertilisation of the eggs using their partner's sperms can be transferred to some other woman who has functioning uterus. hcg
Can you describe the procedure?
You'll first get a class of hormonal drugs to boost your ovaries to produce more follicles (follicles contain eggs). These hormones are generally FSH analogues and they stimulate the ovaries to produce between 50-60 such follicles. The growth of the follicles is going to be monitored closely by testing for hormones and serial ultrasound scans. On around the 10th day an attempt of inject able HCG is given which causes these follicles to mature further. These follicles are then aspirated by a needle passed through the vagina and around 30 of the follicles is going to be aspirated at one time. It can take about 20 min to perform this procedure and during this period women will either be under sedation or general anaesthesia. hcg shots
Once these follicles are aspirated, they're sent to the lab where they will be processed. The eggs are cleaned of the surrounding cells and the very best eggs for fertilisation are selected. They then mixed with washed sperms (sperms that are removed of most inactive cells and seminal fluid) in a petridish and incubated at 37 degree Celsius for 18 hours. During this period the fertilised egg can have two pronuclei.In cases when the sperm has difficulty penetrating the ovum, it could be directly injected into the egg by a process called Intra-cytoplasmic Sperm Injection.Once the eggs are fertilised they're used in a culture chamber where they grow for the next 3-5 days before being implanted in the female. These embryos may be cultured in artificial culture media or endometrial co-culture. In endometrial coculture the embryo is cultured on cells from woman's own endometrium. hcg shots
Anytime 3-5 embryos are placed in the uterus for implantation depending upon many factors.
This is a brief summary about the procedure. You can read the whole article in In Vitro Fertilisation like the success rates, complications and other issues associated with this particular procedure from my website.
